Protein_Domain

Part:BBa_K4803012:Design

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


3xFCS (furin cleavage site)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Major restriction enzyme recognition sequences (EcoRI XbaI SpeI PstI NotI BsaⅠ SapⅠ) are removed from this part. This part was codon optimized for expression in human cell line.

Source

This sequence was designed based on the paper in [1].

Reference

[1] Vlahos, A. E., Kang, J., Aldrete, C. A., Zhu, R., Chong, L. S., Elowitz, M. B., & Gao, X. J. (2022). Protease-controlled secretion and display of intercellular signals. Nature communications, 13(1), 912.